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Article Type

Original Study

Abstract

Objectives The objective of this study was to investigate the in-vitro differentiation of mesenchymal stem cells (MSCs) into cells of the endothelial lineage. Background All tissues depend on the blood supply, and blood supply depends on endothelial cells (ECs). Vascular ECs derived from stem cells could be used in therapeutic strategies for the repair and revascularization of ischemic tissue in patients exhibiting vascular defects. MSCs that can be separated from different tissues have the ability for self-renewal, they can be differentiated into different tissues, and they help in the regeneration of damaged tissue in tissue engineering. Materials and methods This study was carried out on 20 bone marrow samples. A volume of 5 ml of bone marrow was aspirated. Mononuclear cells were separated using Ficoll–Hypaque solution. MSCs were separated from the mononuclear cell fraction using plastic adherence. MSCs were subcultured in 35-mm Petri dishes and then treated with vascular endothelial growth factors. Results MSCs were generated out of mononuclear bone marrow cells. Isolated MSCs were positive for the marker CD44 and negative for CD34. They were able to differentiate into ECs. ECs can be identified by morphology, flow cytometric analysis to detect CD34+, and hematoxylin and eosin staining. Conclusion The present study shows that bone-marrow-derived MSCs can be differentiated in vitro to ECs as future revascularization therapy in ischemic tissue.

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