Article Type

Original Study


Objectives The aim of the study was to evaluate interleukin 10 (IL-10) gene single nucleotide polymorphisms (SNPs) as a susceptibility gene in lepra reactions. Background Leprosy is a chronic dermatoneurological disease, caused by Mycobacterium leprae. Lepra reactions are immune inflammatory-related leprosy phenotypes that may occur before diagnosis of leprosy, during treatment, or after multiple drug therapy. IL-10 gene becomes widely used in studies of immune-mediated disease. It can be a marker of disease susceptibility as well as of disease severity. This work studies IL-10 gene polymorphisms as a susceptibility gene in lepra reactions which may be useful in preventing lepra reactions as a complication of leprosy. Patients and methods This study was conducted on 40 patients with leprosy (20 with reactions), who were recruited from the Menoufia Unit of Dermatology and Leprosy within a period of 1 year, in addition to 20 control subjects, without history of leprosy, from the same geographic area. SNPs –1082A/G, –819C/T, and –592 A/C were identified in cases and control groups by PCR-RFLP. Results The genotype C/C in the SNP-819 was associated with lepra reaction (P < 0.01). There was no association between –592 A/C (P > 0.05) and 1082A/G (P > 0.05) and lepra reaction. The haplotype –1082A–819C–592C/–1082A–819T-592A is considered a risky haplotype category. The study documents that –1082A–819C–592C/–1082A–819C–592C and –1082A–819T–592A/–1082A–819T–592A are considered protective haplotypes. According to the study, the haplotype – 1082G–819C–592C/–1082A–819C–592C has no effect genetically. Conclusion IL-10 gene 819 SNP (C/C) is a risky polymorphism which increases susceptibility to lepra reaction, whereas IL-10 1082 SNP and IL-10 592 SNP are not associated with lepra reactions.