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Subject Area

Pathology

Document Type

Original Study

Abstract

Objectives: to evaluate the role of TP53 gene expression as a potential biomarker for multiple sclerosis. Background: Multiple sclerosis (MS) is the most prevalent non-traumatic illness among young adults. The frequency of MS and its socioeconomic effects are growing globally. Up until now, the diagnosis of MS has been considered a big challenge as it depends on a group of findings known as the McDonald criteria. However, none of those criteria is particularly specific to MS disease. This study aims to evaluate TP53 gene expression as a diagnostic marker for MS. Methods: Twenty-eight MS cases were recruited according to modified McDonald’s criteria (2017). Twenty-eight age- and sex-matched healthy subjects volunteered as a control group. Blood samples were used for RNA. The expression level of the TP53 gene was evaluated by qRT-PCR by TaqMan gene expression assay using a Rotor-Gene Q MDx 5 plex HRM instrument. Results: There was a low expression of TP53 in the MS group in comparison with the control group (P = 0.002). The ROC curve for diagnosis of MS demonstrated the best cutoff value for TP53 RQ was ≤ 0.91, with a sensitivity of 89.3% and specificity of 60.9%. There was no statistically significant correlation between disease duration in years, the expanded disability status scale (EDSS), and the annual relapse rate. Conclusion: TP53 gene expression could be a potential diagnostic biomarker for the diagnosis of MS.

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